GDF-5 (BMP-14/CDMP-1)

GDF-5 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Mutations in this gene are associated with acromesomelic dysplasia, Hunter-Thompson type; brachydactyly, type C; and chondrodysplasia, Grebe type. These associations confirm that the gene product plays a role in skeletal development. GDF-5 produced in E.Coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 120 amino acids and having a total molecular mass of 26.8 Dalton.

GDF5; OS5; LAP4; BMP14; CDMP1; SYNS2

8200

P43026

NP_000548

NM_000557

20q11.2

Human

Human

APLATRQGKR PSKNLKARCS RKALHVNFKD MGWDDWIIAP LEYEAFHCEG LCEFPLRSHL EPTNHAVIQT LMNSMDPEST PPTCCVPTRL SPISILFIDS ANNVVYKQYE DMVVESCGCR

> 98% by SDS-PAGE & RP-HPLC

The ED50 was determined by its ability to induce alkaline phosphatase production by ATDC-5 chondrogenic cells is 1.0-2.0 µg/ml.

120

Lyophilized

26.8 kDa

E. coli