GDF-5 (BMP-14/CDMP-1, Vrij van dieren)

GDF-5 is expressed in long bones during embryonic development and postnatally in articular cartilage. Mutations in the GDF-5 gene have been implicated in Hunter-Thompson type dwarfism and in Grebe Syndrome, which is characterized by short stature, extra digits, and short and deformed extremities. The mature and functional form of GDF-5 is a homodimer of two 120 amino-acid polypeptide chain (monomers) linked by a single disulfide bond. Each GDF-5 monomer is expressed as the C-terminal part of a precursor polypeptide, which also contains a 27 amino acid signal peptide and a 354 amino acid propeptide. This precursor undergoes intracellular dimerization, and upon secretion it is processed by a furin-type protease. Recombinant Human GDF-5 (BMP-14/CDMP-1) is a 27.0 kDa homodimeric disulfide-linked protein consisting of two 120 amino acid polypeptide chains.

Growth/Differentiation Factor-5, Bone Morphogenetic Protein-14, Cartilage-Derived Morphogenetic Protein-1 (CDMP-1) ; GDF5; OS5; LAP4; BMP14; SYNS3

8200

P43026

NP_000548

NM_000557

20q11.2

Human

Human

APLATRQGKR PSKNLKARCS RKALHVNFKD MGWDDWIIAP LEYEAFHCEG LCEFPLRSHL EPTNHAVIQT LMNSMDPEST PPTCCVPTRL SPISILFIDS ANNVVYKQYE DMVVESCGCR

< 0.01 ng/µg of protein (< 0.1 EU/µg)

≥ 98% by SDS-PAGE gel and HPLC analyses.

Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 1.0-2.0 µg /ml.

120

Lyophilized

27 kDa

E.coli