Geitenantimuis IgG (H&L), TRITC-geconjugeerd, min, kruisreactiviteit op serum van runderen, paarden, mensen, varkens of konijnen

Goat anti-mouse IgG (H&L) is a secondary antibody conjugated to TRITC, which binds to mouse IgG (H&L) in immunological assays. Antibody is affinity purified using solid phaseMouse IgG (H&L) . Tetramethylrhodamine-5-isothiocyanate (TRITC) has Amax = 550 nm; Emax = 570 nm.

9007-83-4

38221900

Purified mouse IgG (H&L) AAA51107

Anti-Mouse

The optimal working dilution should be determined by the investigator, Suggested starting dilution (s) : 1 : 20-1 : 2000 for most applications

Immunogen affinity purified goat IgG.

Lyophilized

For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily

Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0) . Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with:heavy chains on mouse IgG, light chains on all mouse immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin mouse serum proteins, serum proteins from bovine, horse, human, pig, or rabbit.

Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.