GENLISA Glycogen Fosforylase b (GPb) APPARAAT Kit

Glycogen Phosphorylase b (GPb) is a key component of cellular glucose metabolism and is routinely studied to understand glycolytic flux, energy balance, and metabolic reprogramming in cancer and immune cells. This microplate-based assay kit is designed for convenient measurement of Glycogen Phosphorylase b (GPb) activity in biological samples such as cell and tissue lysates, cultured cells, and other research matrices. Enzyme activity measurements are useful for pathway mapping, inhibitor screening, functional validation, and comparing metabolic states across experimental conditions. The assay is supplied with ready-to-use reagents and a straightforward workflow that generates a measurable signal proportional to analyte level or activity, compatible with standard microplate readers. Typical applications include metabolic pathway studies, stress-response research, pharmacology/toxicology screening, and functional characterization of engineered cells or disease models. For research use only (RUO) .

Biochemical Assay for the estimation of Glycogen Phosphorylase b (GPb) in Serum, Plasma, Cell Culture Supernatant and other biological samples.

Colorimetric, Absorbance

Colorimetric Method

This Assay is a biochemical based assay adapted to a microwell plate. Based on the biochemical and/or enzymatic reaction, components are added to develop color to estimate the biochemical.

Serum, Plasma, Cell Culture Supernatant and other biological samples.

Shipped at Room Temperature or in gel pack to maintain temperature at 2-8 degrees Celsius

The kit (2-8 Degree Celsius) and its components are to be stored as indicated in the IFU (instructions for use) . To ensure quality of your results, if all the wells in the kit are not being used, store the unused wells in the foil pouch containing the desiccant pack, well sealed and store at the indicated temperature. Similary aliquot and store the reconstituted standard at -20 Degree Celsius for long term storage (maximum 6 months from reconstitution) and to avoid deterioration in the activity.

This assay recognises both the natural and synthetic biochemical / enzyme.