Gepaarde doos 3 Antilichaam / PAX3

Pax genes contain paired domains that share strong homology to genes in Drosophila which are involved in programming early development. The product of the PAX3 gene is a DNA-binding protein expressed during early neurogenesis. Pax-3 is a protein containing both a paired domain and a paired-type homeodomain. During early neurogenesis, Pax-3 expression is limited to mitotic cells in the ventricular zone of the developing spinal cord and to distinct regions in the hindbrain, midbrain and diencephalon. In 10-12 day embryos, expression of Pax-3 is also seen in neural crest cells of the developing spinal ganglia, the craniofacial mesectoderm and in limb mesenchyme. Mutations in the MITF and Pax-3 genes, encoding transcription factors, are responsible for Waardenburg syndrome II (WSII) and WSI/WSIII, respectively.

Immunohistochemistry (FFPE) : 1-2 µg/mL

P23760

Human

A portion of amino acids 150-350 from human PAX3 protein was used as the immunogen for the Paired box 3 antibody.

PAX3/8424

IHC-P

Protein G affinity

Purified

This Paired box 3 antibody is available for research use only.

Aliquot the Paired box 3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

1 mg/mL in 1X PBS; BSA free, sodium azide free

Optimal dilution of the Paired box 3 antibody should be determined by the researcher.

Nucleus

IHC staining of FFPE human tonsil tissue with Paired box 3 antibody (clone PAX3/8424) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.