GFAP Antilichaam / Glial Fibrillair zuur eiwit

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. [UniProt]

Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL

P14136

Human

A recombinant full-length human GFAP protein was used as the immunogen for the GFAP antibody.

GFAP/9424

IHC-P, WB

Protein G affinity

Purified

This GFAP antibody is available for research use only.

Aliquot the GFAP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Optimal dilution of the GFAP antibody should be determined by the researcher.

Cytoplasm

IHC staining of FFPE human brain tissue with GFAP antibody (clone GFAP/9424) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.