GFP-antilichaam [4B10B2]

GFP (Green fluorescence protein) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors. GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.

7011691

B6UPG7

YP_002302326.1

GFP

Ni-NTA purified full-length recombinant GFP-His expressed in E. Coli strain BL21 (DE3) .

4B10B2

Unconjugated

Primary Antibodies

Phospho-Specific

Batch dependent

Purified antibody in PBS containing 0.03% sodium azide.

None

Blue Ice

GFP monoclonal antibody can be stored at -20˚ C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG2a

Gfp

Green fluorescent protein

Neisseria gonorrhoeae

Dittman JS. Kaplan JM. Proc Natl Acad Sci U S A. 2006, Jul 25,103 (30) :11399-404. Epub 2006 Jul 14.

Cavalieri V. Bernardo MD. Spinelli G. Gene Expr Patterns. 2007, Jan,7 (1-2) :124-30. Epub 2006 Jun 14.

Arasada R. Son H. Ramalingam N. et al. Eur J Cell Biol. 2006, Sep,85 (9-10) :1059-68. Epub 2006 Jul 13.

Green fluorescent protein

ELISA, WB

211909965