GFP Antilichaam, Kloon H24: RPE

Green fluorescent protein (GFP) is a 27 kDa reporter protein originally isolated from the jellyfish Aequorea victoria. It emits bright green fluorescence when exposed to ultraviolet or blue light, making it an indispensable tool in molecular and cellular neuroscience. GFP enables real-time visualization of gene expression, protein localization, and cellular dynamics in live cells and tissues without the need for additional substrates or cofactors. In neurodegenerative disease research, GFP is widely used to track neuronal morphology, monitor synaptic changes, and study the progression of protein aggregation in models of Alzheimer’s, Parkinson’s, and Huntington’s disease. By fusing GFP to disease-relevant proteins such as tau, α-synuclein, or huntingtin, researchers can observe pathological processes in real time and assess the efficacy of therapeutic interventions. The recombinant nature of GFP ensures high specificity, consistent expression, and minimal cytotoxicity, which enhances reproducibility across experimental systems. Additionally, its compatibility with advanced imaging techniques—including confocal and two-photon microscopy—makes GFP a cornerstone in the study of neural circuits and neurodegenerative mechanisms. As a genetically encoded fluorescent marker, GFP continues to drive innovation in neuroscience by enabling precise, dynamic, and non-invasive investigation of cellular processes underlying brain function and disease.

Mouse Anti-Jellyfish GFP Recombinant Monoclonal IgG1

GFP, Green fluorescent protein

12352203

Non-hazardous

Non-hazardous

P42212

Fusion protein, AA #1-238 (full length) of jellyfish GFP

H24

RPE

Recombinant Monoclonal

WB | ICC/IF

Tags and Cell Markers | Fluorescent Proteins

Protein A Purified

0.2 µg/ml was sufficient for detection of His-tagged GFP protein

1mg/ml

WB (1:5000), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.; optimal dilutions for assays should be determined by the user.

95.46mM Phosphate, 2.48mM MES and 2mM EDTA

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Blue Ice or 4ºC

Conjugated antibodies should be stored according to the product label

https://www.stressmarq.com/wp-content/uploads/Antibody-MSDS.pdf

Detects full length GFP

1. Prasher, D. C., Eckenrode, V. K., Ward, W. W., Prendergast, F. G., & Cormier, M. J. (1992) . Primary structure of the Aequorea victoria green-fluorescent protein. Gene, 111 (2), 229–233. DOI: 10.1016/0378-1119 (92) 90691-H 2. Tsien, R. Y. (1998) . The green fluorescent protein. Annual Review of Biochemistry, 67 (1), 509–544. DOI: 10.1146/annurev.biochem.67.1.509 3. Bradbury, A., & Plückthun, A. (2015) . Reproducibility: Standardize antibodies used in research. Nature, 518 (7537), 27–29. DOI: 10.1038/518027a

Species Independent

IgG1

Jellyfish