GFP expressie RGD vezel gemodificeerd Adenovirus (Ad (RGD) -CMV-GFP)

Ad infection is initiated by recognition of the native Ad5 receptor, coxsackievirus-adenovirus receptor (CAR), on target cells by the carboxy-terminal portion (i.e., knob) of the fiber protein. Therefore the infection efficiency of adenovirus is very low for cells with low or no CAR receptors. Arg-Gly-Asp (RGD) motif was introduced in the surface-exposed loops of adenovirus fiber knob, this enables the adenovirus to bypass CAR and mediate cell entry via RGD binding integrins. So the RDG modified adenovirus can be used for "retargeting" cells that regular adenovirus doesn't work well. The infection effeciency of RDG modified adenovirus can be tens or hundreds of times higher than standard Ad5 in some cells, including human or mouse macrophages, T cells, synoviocytes, islet grafts, adipocytes, MEF, etc.This is a RGD modified replication-deficient adenovirus expressing eGFP under a CMV promoter.

GFP expressing RGD fiber modified Adenovirus

41106621

3822190000

Pre-Made

Dry Ice

-80C

12 Months

Ad (RGD) -CMV-GFP

Human Adenovirus Type5 (dE1/E3) with RGD-fiber modification

CMV (ubiquitous)

EGFP

DMEM, 2% BSA, 2.5% Glycerol

1x10^10 PFU/ml