GGA2 Antilichaam/Golgi-gelokaliseerd, gamma oorbevattend, ARF-bindend eiwit 2

ELISA: 0.1-0.5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunoprecipitation: 2-4ug/500ug of lysate, Western Blot: 0.25-0.5 µg/mL

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Human

E.coli-derived human GGA2 recombinant protein (Position: L26-A608) was used as the immunogen for the GGA2 antibody.

ELISA, FACS, IP, WB

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This GGA2 antibody is available for research use only.

After reconstitution, the GGA2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the GGA2 antibody should be determined by the researcher.

Flow Cytometry analysis of HepG2 cells using anti-GGA2 antibody. Overlay histogram showing HepG2 cells stained with (Blue line) . To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GGA2 antibody (1 ug/million cells) for 30 min at 20oC. DyLight 488 conjugated goat anti-rabbit IgG (5-10 ug/million cells) was used as secondary antibody for 30 minutes at 20oC. Isotype control antibody (Green line) was rabbit IgG (1 ug/million cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.