GIMAP5 Antilichaam / GTPase IMAP familielid 5

Western Blot: 0.25-0.5 µg/mL, ELISA: 0.1-0.5 µg/mL

Q96F15

Human

E.coli-derived human GIMAP5 recombinant protein (Position: M1-E240) was used as the immunogen for the GIMAP5 antibody.

WB, ELISA

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This GIMAP5 antibody is available for research use only.

After reconstitution, the GIMAP5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the GIMAP5 antibody should be determined by the researcher.

Western blot analysis of GIMAP5 using anti-GIMAP5 antibody. Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human THP-1 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GIMAP5 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. The expected band size for GIMAP5 is at 35 kDa. With a lymphocyte-specific expression pattern, GIMAP5 should not be observed in HeLa cells (epithelial) .