GIPC1 Antilichaam/PDZ domein-bevattend eiwit GIPC1

Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

O14908

Human, Mouse, Rat

E.coli-derived human GIPC1 recombinant protein (Position: H66-Y333) was used as the immunogen for the GIPC1 antibody.

WB, IHC, ICC, IF, FACS, ELISA

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This GIPC1 antibody is available for research use only.

After reconstitution, the GIPC1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the GIPC1 antibody should be determined by the researcher.

Cytoplasm, cell membrane, nucleus

Immunofluorescent staining of GIPC1 using anti-GIPC1 antibody (red) . GIPC1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-GIPC1 antibody overnight at 4oC. DyLight 594 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue) . The tissue section was developed using Phalloidin-iFluor 488 Conjugated (green) . Visualize using a fluorescence microscope and filter sets appropriate for the label used.