Glutaminase Antilichaam / GLS

Sahai (1983) demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter (Prusiner, 1981) . High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.

Western blot: 1:1000-1:2000, Immunofluorescence: 1:25, Immunohistochemistry (FFPE) : 1:100-1:200, Flow cytometry: 1:25 (per 1x10e6 cells)

O94925

Human, Mouse, Rat

A portion of amino acids 516-545 from the human protein were used as the immunogen for the Glutaminase antibody.

WB, IHC-P, FACS, IF

Antigen affinity purified

Purified

This Glutaminase antibody is available for research use only.

Aliquot the Glutaminase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

In 1X PBS, pH 7.4, with 0.09% sodium azide

The stated application concentrations are suggested starting points. Titration of the Glutaminase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Cytoplasmic

Immunofluorescent staining of fixed and permeabilized human HepG2 cells with Glutaminase antibody (green), DAPI nuclear stain (blue) and anti-Actin (red) .