Glutathion Reductase Antilichaam / GSR

Glutathione reductase (GR), also known as glutathione-disulfide reductase (GSR), is an enzyme that in humans is encoded by the GSR gene. This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. This enzyme is a homodimeric flavoprotein. It is a central enzyme of cellular antioxidant defense, and reduces oxidized glutathione disulfide (GSSG) to the sulfhydryl form GSH, which is an important cellular antioxidant. Rare mutations in this gene result in hereditary glutathione reductase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been found.

Western blot: 0.5-1 µg/mL, Immunofluorescence (FFPE) : 5 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/10^6 cells, Direct ELISA: 0.1-0.5 µg/mL

P00390

Human, Mouse, Rat

A recombinant human protein corresponding to amino acids K256-R522 was used as the immunogen for the Glutathione Reductase antibody.

WB, FACS, IHC-P, IF, ELISA

Antigen affinity purified

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Glutathione Reductase antibody is available for research use only.

After reconstitution, the Glutathione Reductase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Glutathione Reductase antibody should be determined by the researcher.

Cytoplasm

Western blot testing of human 1) HeLa, 2) HepG2, 3) A549 and 4) 22RV1 cell lysate with Glutathione Reductase antibody at 0.5ug/ml. Predicted molecular weight ~55 kDa.