GMFB cDNA

GMFB, also known as GMF, belongs to the GMF subfamily of the larger actin-binding protein ADF family. This protein, which is phosphorylated following phorbol ester stimulation, is important for the nervous system. It causes brain cell differentiation, stimulates neural regeneration and inhibits tumor cell proliferation. Overexpression of GMFB in astrocytes has been shown to enhance brain-derived neurotrohic factor (BDNF) production. GMFB expression is increased by exercise, and the protein is crucial for exercise-induction of BDNF.

GMF

14q22.2

Human

PATGen (puc19-derived cloning vector)

ATGAGTGAGT CTTTGGTTGT TTGTGATGTT GCCGAAGATT TAGTGGAAAA GCTGAGAAAG TTTCGTTTTC GCAAAGAAAC GAACAACGCT GCTATTATAA TGAAGATTGA CAAGGATAAA CGCCTGGTGG TACTGGATGA GGAGCTTGAG GGCATTTCAC CAGATGAACT TAAAGATGAA CTACCTGAAC GACAACCTCG CTTCATTGTG TATAGTTATA AATATCAACA TGATGATGGA AGAGTTTCAT ATCCTCTGTG CTTTATTTTC TCCAGTCCTG TTGGATGTAA GCCTGAACAA CAGATGATGT ATGCTGGAAG TAAGAATAAG CTAGTCCAGA CAGCTGAACT AACCAAGGTA TTTGAAATAA GAAATACCGA AGACCTAACT GAAGAATGGT TACGTGAGAA ACTTGGATTT TTTCACTAA

GMFB, GMF, GMFB, ATGD0058-10 µg, ATGD0058-20 µg, ATGD0058-50 µg, ATGD0058-100 µg, ATGD0058-250 µg, ATGD0058-500 µg, ATGD0058-1 mg, ATGD0058-10, ATGD0058-20, ATGD0058-50, ATGD0058-100, ATGD0058-250, ATGD0058-500, ATGD0058-1

Store the plasmid at -20C.

Lyophilized

Neuroscience

601713

NP_004115.1

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_004124.2

429bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.