GP2 Recombinant eiwit

Source : Sf9 insect cells. GP2 is a cDNA coding for the free form of the human pancreatic secretory granule membrane major glycoprotein / GP2 having a molecular mass of 54,909 Dalton (protein component excluding glycosylation; observed molecular weight approx. 68 kDa) pH 5.4. GP2 protein is fused to a deca-histidine purification tag. Crohn's Disease (CD), which is very common in Caucasians, is an inflammatory bowel diseases (IBD) . Mucosal inflammation in CD seems to arise when dysregulation of the immune system breaks the balance between tolerance to commensal microbiota or food-derived antigens and immunity to pathogens. The autoimmune mechanisms takes part in the development of CD, and exocrine pancreas autoantibodies (PAB) are disease-specific in CD patients. It was established not too long ago that glycoprotein 2 (GP2) is the major autoantigenic mark recognized by CD-specific PAB. Furthermore to IgG and IgM PAB isotypes, IgA pancreatic autoantibodies were also identified in CD patients. GP2 is an extremely glycosylated 78 kDa protein with N-linked carbohydrates. It is responsible for up to 40% of all zymogen granule (ZG) membrane proteins in pancreatic acinar cells and is linked to the ZG membrane via a glycosyl phosphoinositol (GPI) anchor.

Glycoprotein 2 (zymogen granule membrane) ||Pancreatic zymogen granule membrane protein GP-2||ZAP75||DKFZp779K0533.

Greater than 80% as determined by SDS-PAGE.

GP2 solution is supplied in 16mM HEPES buffer pH-7.5, 80mM NaCl and 20% glycerol.

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.