GPNMB Antilichaam / Transmembrane glycoproteïne NMB / HGFN

Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

Q99P91

Human, Mouse, Rat

E.coli-derived mouse GPNMB recombinant protein (Position: R164-D564) was used as the immunogen for the GPNMB antibody.

WB, IHC, ICC, IF, FACS, ELISA

Immunogen affinity purified

Lyophilized

243

This GPNMB antibody is available for research use only.

After reconstitution, the GPNMB antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the GPNMB antibody should be determined by the researcher.

Cytoplasm, cell membrane

Immunohistochemical staining of GPNMB using anti-GPNMB antibody. GPNMB was detected in paraffin-embedded section of human liver cancer. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GPNMB antibody overnight at 4oC. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.