Groei Hormone, placenta 20K

Growth Hormone (GH), also known as somatotropin, is a pleiotropic cytokine of the hematopoietic growth factor superfamily, which encompasses most cytokines, hematopoietic growth factors, and related receptors, and includes the related growth hormone receptor, prolactin, placental lactogens, proliferins, and somatolactin (SST) . GH is primarily recognized for its anabolic role in stimulating the growth and differentiation of muscle, bone, and cartilage. A number of other functions, including immunomodulatory actions, are also attributed to GH, due in part to the pervasive distribution of its receptors, and the indirect effects associated with GH-stimulated production of insulin-like growth factors (IGFs) . Occurring predominantly in the somatotropes of the anterior pituitary, whereupon it is stored in secretory granules, production of GH has also been noted in many other tissues, including those of the hematopoietic system. The production and pulsatile release of circulating GH is very tightly regulated by both negative and positive feedback regulations of pituitary and hypothalamic hormones, such as Pituitary-specific Positive Transcription Factor 1 (POU1F1), Growth Hormone Releasing Hormone (GHRH), and somatostatin (SRIF) . Deficient production of GH is associated with dwarfism and reduction of lean body mass, while overproduction is associated with acromegaly and gigantism, as well as breast tumor growth. Recombinant Human placental Growth Hormone K20 is a 20.4 kDa, single, non-glycosylated polypeptide chain containing 177 amino acid residues. Placental Growth Hormone 20K is devoid of lactogenic (prolactin receptor mediated) activity characteristic to pituitary GHs.

Growth hormone variant, GH-V, Growth hormone 2, Placenta-specific growth hormone

2689

P01242-1

NP_002050.1

NM_002059.5

Human

Human

AFPTIPLSRL FDNAMLRARR LYQLAYDTYQ EFNPQTSLCF SESIPTPSNR VKTQQKSNLE LLRISLLLIQ SWLEPVQLLR SVFANSLVYG ASDSNVYRHL KDLEEGIQTL MWRLEDGSPR TGQIFNQSYS KFDTKSHNDD ALLKNYGLLY CFRKDMDKVE TFLRIVQCRS VEGSCGF

It is recommended to reconstitute the lyophilized rHuGH-20K-pl in 0.4% NaHCO3 or water adjusted to pH 11, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

< 0.1 ng/µg of protein (< 1EU/µg)

> 98.0% as determined by RP-HPLC, Gel filtration and SDS-PAGE Silver Stained gel.

RHuGH-20K-pl is fully biologically active when compared to WHO reference standard using in vitro bioassay. It is also capable of forming a 1:2 complex with the recombinant human growth hormone receptor extracellular domain (ECD) .

177

Lyophilized

Water, pH 11

20.4 kDa

E. coli

AFPTI