Groeihormoonmutant (G119R)

Recombinant zebrafish growth hormone mutant G119R (zbGH mutant) produced in E.Coli (22 K) is a single, non-glycosylated, polypeptide chain containing 185 amino acids with an additional Ala at its N-terminus and having a molecular mass of 21187 Dalton.

Somatotropin, GH, GH-N, Growth Hormone 1, Pituitary growth hormone

407639

Q1JQ34

NP_001018328.2

NM_001020492.2

Zebrafish

AQRLFNNAVIRVQHLHQLAAKMINDFEEGLMPEERRQLSKIFPLSFCNSDSIETPTGKDETQKSSMLKLLRISFRLIESWEFPSQTLSSTISNSLTIGNPNQITEKLADLKMGISVLIKRCLDGQPNMDDNDSLPLPFEDFYLTVGETSLRESFRLLACFKKDMHKVETYLRVANCRRSLDSNCTL

It is recommended to reconstitute the lyophilized zfGH mutant in sterile water or 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml and not more than 3 mg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.

< 0.1 ng/µg of protein (< 1EU/µg)

> 99.0% as determined by RP-HPLC, Gel filtration and SDS-PAGE Silver Stained gel.

The biologically activity of zfGH mutant in PDF-P1 3B9 cells stably transfected with rabbit GH receptors has not yet been tested.

186

Lyophilized

Water

21.1 kDa

E. coli

AQRLFN