GST-antilichaam [6G9B9]

Plasmid vectors for the expression of coding regions of eukaryotic genes in E. coliare in common usage; such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. For instance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase (GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. coli.

12931264

P0A9D2

P0A9D2

GST

Ni-NTA purified recombinant GST expressed in E. Coli strain M15.

6G9B9

Unconjugated

Primary Antibodies

Other

Antibody is purified by protein A affinity chromatography.

Batch dependent

Purified antibody in PBS containing 0.03% sodium azide.

None

Blue Ice

GST monoclonal antibody can be stored at -20˚ C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG1

Gst

Glutathionine S-transferase

Escherichia coli str. K-12 substr. W3110

Singlestep purification of polypeptides expressed in Escherichia colias fusions with glutathione Stransferase. 1988. Gene 67: 31-40.

Gst, b1635, JW1627, GST B1-1

ELISA, WB

71159393