GTF2E1 Antilichaam / TFIIE alfa

Immunofluorescence: 1:50-1:200, Immunohistochemistry: 1:50-1:200, Immunocytochemistry/Western blot: 1:500-1:2000

P29083

Human, Mouse, Rat

A synthesized peptide derived from human TFIIE alpha was used as the immunogen for the GTF2E1 antibody.

31G53

IF, IHC, ICC, WB

Affinity chromatography

Liquid

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

This GTF2E1 antibody is available for research use only.

Store the GTF2E1 antibody at -20°C.

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Optimal dilution of the GTF2E1 antibody should be determined by the researcher.

Western blot testing of human samples using the GTF2E1 antibody at 1:1000 dilution for 1 hour at room temperature. A dominant band is detected at ~60-65 kDa with a second band at ~50-55 kDa, although the calculated molecular weight is ~49 kDa. The higher-migrating species is consistent with hyper-phosphorylated TFIIE alpha during transcription initiation, while the lower band represents a less-phosphorylated form.