H-2Db Antilichaam [28-14-8]

The “classical” MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant beta2-microglobulin. These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes which are usually cytotoxic T cells. MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny. The monoclonal antibody 28-14-8 binds to the alpha3 domain of H-2Db in the presence or absence of beta2 microglobulin. It cross reacts with the alpha3 domain of H-2Ld, but not Kd or Dd, and with H-2Dq and/or Lq. The antibody has been shown to block H-2Ld-specific and H-2Ld-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes but it does not block recognition of H-2Ld-positive target cells by Ly-6G2-positive NK cells.

14964

P01899

P01899

Mouse

C3H.SW mouse splenocytes

[28-14-8]

Unconjugated

Primary Antibodies

Immunology

0.5 mg/mL

Supplied in PBS

None

Dry Ice

Store vial at -20˚ C

IgG2a

H-2Db

H-2Db Antibody [28-14-8] for use in flow cytometry, immunohistochemistry, immunoprecipitation, CMCD, and blocking assays.

H2-D1

Histocompatibility 2, D region locus 1

Mus musculus

MHC Class I

Flow

Liquid