HaCaT Lysate

HaCaT cells are spontaneously transformed aneuploid immortal keratinocyte cell line from adult human skin. HaCaT cells are utilized for their high capacity to differentiate and proliferate in vitro. Their use in research allows for the characterization of human keratinocyte using a model that is reproducible and addresses issues such as short culture lifespan and variations between cell lines that would otherwise be encountered.

Human

WB

2 mg/mL (determined by BCA assay)

HaCaT, ATGL0015-10 µg, ATGL0015-20 µg, ATGL0015-50 µg, ATGL0015-100 µg, ATGL0015-250 µg, ATGL0015-500 µg, ATGL0015-1 mg, ATGL0015-10, ATGL0015-20, ATGL0015-50, ATGL0015-100, ATGL0015-250, ATGL0015-500, ATGL0015-1

Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.

Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)

Lysates

Human

All lanes : HaCaT Whole Cell Lysate (40ug) ; ; ; ; Lane 1. : Anti-GAPDH antibody (ATGA0181) ; ; Lane 2. : Anti-Beta tubulin antibody (ATGA0196) ;

Heat lysate to 95°C for 5 minutes and rapidly cooling before use.

Western Blot analyzed by both GAPDH and Beta tubulin antibody