HADH Antilichaam / Hydroxyacyl-CoA Dehydrogenase / HAD1

Hydroxyacyl-Coenzyme A dehydrogenase (HADH) is an enzyme which in humans is encoded by the HADH gene. This gene is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene of this gene on chromosome 15.

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

Q16836

Human, Mouse, Rat

An E.coli-derived human recombinant protein (amino acids A43-K301) was used as the immunogen for the HADH antibody.

WB, IHC-P, IF, FACS, ELISA

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This HADH antibody is available for research use only.

After reconstitution, the HADH Antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the HADH antibody should be determined by the researcher.

Cytoplasm

IHC staining of FFPE human liver cancer tissue with HADH antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.