HAND1 cDNA

HAND1 belongs to the basic helix-loop-helix family of transcription factors. This protein is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, it has been suggested that this transcription factor may be required for early trophoblast differentiation

BHLHa27, eHand, Hxt, Thing1

5q33

Human

PATGen (puc19-derived cloning vector)

ATGAACCTCG TGGGCAGCTA CGCACACCAT CACCACCATC ACCACCCGCA CCCTGCGCAC CCCATGCTCC ACGAACCCTT CCTCTTCGGT CCGGCCTCGC GCTGTCATCA GGAAAGGCCC TACTTCCAGA GCTGGCTGCT GAGCCCGGCT GACGCTGCCC CGGACTTCCC TGCGGGCGGG CCGCCGCCCG CGGCCGCTGC AGCCGCCACC GCCTATGGTC CTGACGCCAG GCCTGGGCAG AGCCCCGGGC GGCTGGAGGC GCTTGGCGGC CGTCTTGGCC GGCGGAAAGG CTCAGGACCC AAGAAGGAGC GGAGACGCAC TGAGAGCATT AACAGCGCAT TCGCGGAGTT GCGCGAGTGC ATCCCCAACG TGCCGGCCGA CACCAAGCTC TCCAAGATCA AGACTCTGCG CCTAGCCACC AGCTACATCG CCTACCTGAT GGACGTGCTG GCCAAGGATG CACAGTCTGG CGATCCCGAG GCCTTCAAGG CTGAACTCAA GAAGGCGGAT GGCGGCCGTG AGAGCAAGCG GAAAAGGGAG CTGCAGCAGC ACGAAGGTTT TCCTCCTGCC CTGGGCCCAG TCGAGAAGAG GATTAAAGGA CGCACCGGCT GGCCGCAGCA AGTCTGGGCG CTGGAGTTAA ACCAGTGA

HAND1, bHLHa27, eHand, Hxt, Thing1, HAND1, ATGD0017-10 µg, ATGD0017-20 µg, ATGD0017-50 µg, ATGD0017-100 µg, ATGD0017-250 µg, ATGD0017-500 µg, ATGD0017-1 mg, ATGD0017-10, ATGD0017-20, ATGD0017-50, ATGD0017-100, ATGD0017-250, ATGD0017-500, ATGD0017-1

Store the plasmid at -20C.

Lyophilized

Development

602406

NP_004812.1

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_004821.2

648bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.