HB-EGF

HB-EGF is an EGF related growth factor that signals through the EGF receptor, and stimulates the proliferation of smooth muscle cells (SMC), fibroblasts, epithelial cells, and keratinocytes. HB-EGF is expressed in numerous cell types and tissues, including vascular endothelial cells and SMC, macrophages, skeletal muscle, keratinocytes, and certain tumor cells. The ability of HB-EGF to specifically bind heparin and heparin sulfate proteoglycans is distinct from other EGF-like molecules, and may be related to the enhanced mitogenic activity, relative to EGF, that HB-EGF exerts on smooth muscle cells. The human HB-EGF gene encodes a 208 amino acid transmembrane protein, which can be proteolytically cleaved to produce soluble HB-EGF. Recombinant human HB-EGF is a 9.7 kDa protein containing 86 amino acid residues, corresponding to the extra-cellular EGF-like and heparin binding domains of the full length HB-EGF protein.

HBEGF; DTR; DTS; DTSF; HEGFL

1839

Q99075

NP_001936.1

NM_001945

5q23

Human

Human, Mouse, Rat

DLQEADLDLL RVTLSSKPQA LATPNKEEHG KRKKKGKGLG KKRDPCLRKY KDFCIHGECK YVKELRAPSC ICHPGYHGER CHGLSL

< 0.1 ng/µg of protein (< 1EU/µg)

> 95% by SDS-PAGE & HPLC analyses

The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.

86

Lyophilized

9.7 kDa

E. coli