HBP1 Antilichaam / HMG-box transcriptiefactor 1

Western Blot: 0.25-0.5 µg/mL, ELISA: 0.1-0.5 µg/mL

O60381

Human, Mouse, Rat

E.coli-derived human HBP1 recombinant protein (Position: D51-R506) was used as the immunogen for the HBP1 antibody.

WB, ELISA

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This HBP1 antibody is available for research use only.

After reconstitution, the HBP1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the HBP1 antibody should be determined by the researcher.

Western blot analysis of HBP1 using anti-HBP1 antibody. Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human K562 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human SIHA whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HBP1 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. HBP1 western blot shows a predominant band at ~70 kDa. Although the predicted size is ~58 kDa, HBP1 frequently migrates at ~68-72 kDa due to its acidic, low-complexity regions and phosphorylation-dependent mobility.