HBZ Antilichaam / Hemoglobine zeta

Western Blot: 0.25-0.5 µg/mL, ELISA: 0.1-0.5 µg/mL

P02008

Mouse, Rat

E.coli-derived human HBZ recombinant protein (Position: D48-R142) was used as the immunogen for the HBZ antibody.

WB, ELISA

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This HBZ antibody is available for research use only.

After reconstitution, the HBZ antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the HBZ antibody should be determined by the researcher.

Western blot analysis of HBZ using anti-HBZ antibody. Lane 1: rat bone tissue lysates, Lane 2: mouse bone tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HBZ antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. Expected molecular weight of Hemoglobin subunit zeta (HBZ) ~16 kDa (142 aa) . On SDS-PAGE/Western blot, HBZ typically migrates at ~13–14 kDa due to its compact globin structure and atypical SDS binding. The observed band just below the 15 kDa marker is consistent with published reports for zeta-globin.