HeLa Lysate

HeLa cells are human epithelial cells from a fatal cervical cancer cells taken from Henrietta Lacks, in 1951. The cell line was found to be remarkably durable and prolific as illustrated by its contamination of many other cell lines used in research. Horizontal gene transfer from human papillomavirus 18 (HPV18) to human cervical cells created the HeLa genome which is different from either parent genome in various ways including its number of chromosomes.

Human

WB

2 mg/mL (determined by BCA assay)

HeLa, ATGL0001-10 µg, ATGL0001-20 µg, ATGL0001-50 µg, ATGL0001-100 µg, ATGL0001-250 µg, ATGL0001-500 µg, ATGL0001-1 mg, ATGL0001-10, ATGL0001-20, ATGL0001-50, ATGL0001-100, ATGL0001-250, ATGL0001-500, ATGL0001-1

Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.

Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)

Lysates

Human

All lanes : HeLa Whole Cell Lysate (40ug) ; ; ; ; Lane 1. : Anti-GAPDH antibody (ATGA0181) ; ; Lane 2. : Anti-Beta tubulin antibody (ATGA0196) ;

Heat lysate to 95°C for 5 minutes and rapidly cooling before use.

Western Blot analyzed by both GAPDH and Beta tubulin antibody