HepG2 Lysaat

HepG2 cells are immortalized cell line that derived from liver tissue of a 15-year-old boy with hepatocellular carcinoma. HepG2 cells secrete a variety of major plasma proteins such as albumin, transferrin, and secrete acute-phase proteins such as fibrinogen, alpha 2-macroglobulin, alpha 1-antitrypsin, transferrin, and plasminogen. HepG2 have an epithelial morphology and are thought to be a very useful model to study HBV virus replication via transfection. Cells are also used for cancer and apoptosis studies.

Human

WB

2 mg/mL (determined by BCA assay)

HepG2, ATGL0005-10 µg, ATGL0005-20 µg, ATGL0005-50 µg, ATGL0005-100 µg, ATGL0005-250 µg, ATGL0005-500 µg, ATGL0005-1 mg, ATGL0005-10, ATGL0005-20, ATGL0005-50, ATGL0005-100, ATGL0005-250, ATGL0005-500, ATGL0005-1

Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.

Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)

Lysates

Human

All lanes : HepG2 Whole Cell Lysate (40ug) ; ; ; ; Lane 1. : Anti-GAPDH antibody (ATGA0181) ; ; Lane 2. : Anti-Beta tubulin antibody (ATGA0196)

Heat lysate to 95°C for 5 minutes and rapidly cooling before use.

Western Blot analyzed by both GAPDH and Beta tubulin antibody