HET

IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes. Recombinant human IL-2 (modified at the C-terminal end) is a 15.5 kDa protein, containing 156 amino acid residues including one intrachain disulfide bond.

IL2; IL-2; TCGF; lymphokine

3558

P60568

NP_000577

NM_000586

4q26-q27

Human

Human

MAPTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFCQSIISTLHYKDPAANKARKEAELAAATAEQ

The lyophilized IL-2 should be reconstituted in 50mM acetic acid to a concentration not less than 100µg/ml. This solution can be diluted into other buffered solutions or stored at -20°C for future use.

< 0.1 ng per µg of IL-2

> 98% by SDS-PAGE

The ED50 as determined by the dose-dependent stimulation of proliferation of murine D10G4.1 cells is in the range of 0.1-0.5 ng/ml.

156

Lyophilized

50mM acetic acid

50mM acetic acid

15.5 kDa

The lyophilized IL-2, though stable at room temperature, is best stored desiccated below 0°C. Reconstituted IL-2 should be stored in workingaliquots at -20°C. Avoid repeated freeze-thaw cycles.

E. coli

APTSSSTK and MAPTSSST