HEXIM2 Antilichaam / Hexamethyleen bisaceetamide-induceerbaar eiwit 2

Western Blot: 0.25-0.5 µg/mL, ELISA: 0.1-0.5 µg/mL

Q3TVI4

Human, Mouse, Rat

E.coli-derived mouse HEXIM2 recombinant protein (Position: L17-R313) was used as the immunogen for the HEXIM2 antibody.

WB, ELISA

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This HEXIM2 antibody is available for research use only.

After reconstitution, the HEXIM2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the HEXIM2 antibody should be determined by the researcher.

Western blot analysis of HEXIM2 using anti-HEXIM2 antibody. Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: rat testis tissue lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse TM4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HEXIM2 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. Western blot detection of HEXIM2 shows a single band at ~38 kDa in rat and ~40 kDa in mouse samples. Despite a calculated mass of ~32-33 kDa, HEXIM2 frequently migrates larger on SDS-PAGE, consistent with its acidic regions and post-translationally modified forms.