Hexokinase 1 Antilichaam / HK1

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific.

Western blot: 2-4 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

P19367

Human

A portion of amino acids 1-200 from human HK1 protein was used as the immunogen for the Hexokinase 1 antibody.

HK1/9510

WB, IHC-P

Protein G affinity

Purified

This Hexokinase 1 antibody is available for research use only.

Aliquot the Hexokinase 1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Optimal dilution of the Hexokinase 1 antibody should be determined by the researcher.

IHC staining of FFPE human brain tissue with Hexokinase 1 antibody (clone HK1/9510) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.