Histone H2AX Antilichaam

H2A histone family member X (usually abbreviated as H2AX) is a type of histone protein from theH2A family encoded by the H2AFX gene. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunoprecipitation: 2ug/500ug of lysate, Direct ELISA: 0.1-0.5 µg/mL

P16104

Human, Mouse, Rat

Amino acids S2-T121 from the human protein were used as the immunogen for the Histone H2AX antibody.

WB, IHC-P, FACS, IP, Direct ELISA

Affinity purified

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This Histone H2AX antibody is available for research use only.

Store the Histone H2AX antibody at -20°C.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the Histone H2AX antibody should be determined by the researcher.

IHC staining of FFPE human breast cancer tissue with Histone H2AX antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.