Histone H3 (acetyl K14) Antilichaam / HIST1H3A

Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Immunoprecipitation: 2-4ug/500ug of lysate

P68431

Human, Mouse, Rat

A synthesized peptide derived from human Histone H3 (acetyl K14) was used as the immunogen for the Histone H3 (acetyl K14) antibody.

BID-8

WB, IHC, ICC, IF, IP

Affinity-chromatography

Liquid

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

This Histone H3 (acetyl K14) antibody is available for research use only.

Store the Histone H3 (acetyl K14) antibody at -20°C.

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Optimal dilution of the Histone H3 (acetyl K14) antibody should be determined by the researcher.

Nuclear

IHC analysis of Histone H3 (acetyl K14) using anti-Histone H3 (acetyl K14) antibody. Histone H3 (acetyl K14) was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with a dilution of 1:50 rabbit anti-Histone H3 (acetyl K14) antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.