Histone H3 (acetyl K9) Antilichaam / HIST1H3A

Western blot: 1:500-1:2000, Immunohistochemistry: 1:50-1:200, Immunocytochemistry/Immunofluorescence: 1:50-1:200, Immunoprecipitation: 1:50, Flow Cytometry: 1:50

P68431

Human, Mouse, Rat

A synthesized peptide derived from human Histone H3 (acetyl K9) was used as the immunogen for the Histone H3 (acetyl K9) antibody.

32H23

WB, IHC, ICC, IF, IP, FACS

Affinity chromatography

Liquid

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

This Histone H3 (acetyl K9) antibody is available for research use only.

Store the Histone H3 (acetyl K9) antibody at -20°C.

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Optimal dilution of the Histone H3 (acetyl K9) antibody should be determined by the researcher.

Nuclear

Western blot analysis of Histone H3 (acetyl K9) using anti-Histone H3 (acetyl K9) antibody. Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human 293T whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human T98G whole cell lysates, Lane 4: human whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human SH-SY5Y whole cell lysates, Lane 7: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Histone H3 (acetyl K9) antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. A specific band was detected for Histone H3 (acetyl K9) at approximately 15 kDa. The expected molecular weight of Histone H3 (acetyl K9) is at 15 kDa.