Histone H3 (acetyl K9) Antilichaam / HIST1H3A

Western blot: 1:500-1:2000, Immunohistochemistry: 1:50-1:200, Immunocytochemistry/Immunofluorescence: 1:50-1:200

P68431

Human, Mouse

A synthesized peptide derived from human Histone H3 (acetyl K9) was used as the immunogen for the Histone H3 (acetyl K9) antibody.

32H22

WB, IHC, ICC, IF

Affinity-chromatography

Liquid

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

This Histone H3 (acetyl K9) antibody is available for research use only.

Store the Histone H3 (acetyl K9) antibody at -20°C.

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Optimal dilution of the Histone H3 (acetyl K9) antibody should be determined by the researcher.

Immunohistochemical staining of Histone H3 (acetyl K9) using anti-Histone H3 (acetyl K9) antibody. Histone H3 (acetyl K9) was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with a dilution of 1:50 rabbit anti-Histone H3 (acetyl K9) antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.