Histone H3 Antilichaam / HIST1H3A/B/C/D/E/F/G/H/I/J

ELISA: 0.1-0.5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunocytochemistry: 5 µg/mL, Western Blot: 0.25-0.5 µg/mL

P68431

Human, Mouse, Rat

E.coli-derived human Histone H3 recombinant protein (Position: Q56�R117) was used as the immunogen for the Histone H3 antibody.

ELISA, FACS, IF, IHC, ICC, WB

Immunogen affinity purified

Lyophilized

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.

This Histone H3 antibody is available for research use only.

After reconstitution, the Histone H3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the Histone H3 antibody should be determined by the researcher.

Western blot analysis of Histone H3 using anti-Histone H3 antibody. Lane 1: rat brain tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysates, Lane 8: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Histone H3 antibody at 0.25 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A specific band was detected for Histone H3 at approximately 17 kDa. The expected molecular weight of Histone H3 is at 15 kDa.