HLA-E Antilichaam/MHC-klasse I-antigeen E

Major histocompatibility complex (MHC) molecules, which include human leukocyte antigens (HLAs), form an integral part of the immune response system. They are cell-surface receptors that bind foreign peptides and present them to cytotoxic T lymphocytes (CTLs) . MHC class I molecules consist of two polypeptide chains, an a or heavy chain and a non-covalently associated protein, b-2-Microglobulin. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes. HLA-A is a MHC class I heavy chain molecule that plays a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. HLA-B and HLA-C are proteins encoded by closely related genes that also exist in the MHC class I. HLA-E belongs to the HLA class I heavy chain paralogs. HLA-E is a heterodimer consisting of a heavy chain and a light chain. The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules.

Immunohistochemistry (FFPE) : 1-2 µg/mL

P13747

Human

A portion of amino acids 1-150 from human HLAE protein was used as the immunogen for the HLA-E antibody.

HLAE/9468

IHC-P

Protein G affinity

Purified

This HLA-E antibody is available for research use only.

Aliquot the HLA-E antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Optimal dilution of the HLA-E antibody should be determined by the researcher.

Secreted

IHC staining of FFPE human tonsil tissue with HLA-E antibody (clone HLAE/9468) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.