HOMER1 Antilichaam / SYN47

Western blot: 1:500-1:2000, Immunohistochemistry: 1:50-1:200, Immunoprecipitation: 1:50

Q86YM7

Human, Mouse, Rat

A synthesized peptide derived from human Homer1 was used as the immunogen for the HOMER1 antibody.

30H34

WB, IHC, IP

Affinity chromatography

Liquid

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

This HOMER1 antibody is available for research use only.

Store the HOMER1 antibody at -20°C.

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Optimal dilution of the HOMER1 antibody should be determined by the researcher.

Western blot analysis of HOMER1 using anti-HOMER1 antibody. Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: rat brain tissue lysates, Lane 2: rat skeletal muscle tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOMER1 antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. A predominant band is detected between an approximately 45 and 50 kDa in all samples, running above the predicted ~40 kDa mass but consistent with the higher apparent molecular weight of the long HOMER1b/c isoforms, which are strongly enriched in brain.