HPGD cDNA

HPGD encodes a member of the short-chain nonmetalloenzyme alcohol dehydrogenase protein family. The encoded enzyme is responsible for the metabolism of prostaglandins, which function in a variety of physiologic and cellular processes such as inflammation. Mutations in this gene result in primary autosomal recessive hypertrophic osteoarthropathy and cranioosteoarthropathy. Multiple transcript variants encoding different isoforms have been found for this gene.

15-hydroxyprostaglandin dehydrogenase [NAD+], 15-PGDH, PGDH, PGDH1, SDR36C1, Prostaglandin dehydrogenase 1

4q34-q35

Human

PATGen (puc19-derived cloning vector)

ATGCACGTGAACGGCAAAGTGGCGCTGGTGACCGGCGCGGCTCAGGGCATAGGCAGAGCCTTTGCAGAGGCGCTGCTGCTTAAGGGCGCCAAGGTAGCGCTGGTGGATTGGAATCTTGAAGCAGGTGTACAGTGTAAAGCTGCCCTGGATGAGCAGTTTGAACCTCAGAAGACTCTGTTCATCCAGTGCGATGTGGCTGACCAGCAACAACTGAGAGACACTTTTAGAAAAGTTGTAGACCACTTTGGAAGACTGGACATTTTGGTCAATAATGCTGGAGTGAATAATGAGAAAAACTGGGAAAAAACTCTGCAAATTAATTTGGTTTCTGTTATCAGTGGAACCTATCTTGGTTTGGATTACATGAGTAAGCAAAATGGAGGTGAAGGCGGCATCATTATCAATATGTCATCTTTAGCAGGACTCATGCCCGTTGCACAGCAGCCGGTTTATTGTGCTTCAAAGCATGGCATAGTTGGATTCACACGCTCAGCAGCGTTGGCTGCTAATCTTATGAACAGTGGTGTGAGACTGAATGCCATTTGTCCAGGCTTTGTTAACACAGCCATCCTTGAATCAATTGAAAAAGAAGAAAACATGGGACAATATATAGAATATAAGGATCATATCAAGGATATGATTAAATACTATGGAATTTTGGACCCACCATTGATTGCCAATGGATTGATAACACTCATTGAAGATGATGCTTTAAATGGTGCTATTATGAAGATCACAACTTCTAAGGGAATTCATTTTCAAGACTATGATACAACTCCATTTCAAGCAAAAACCCAATGA

15 Hydroxyprostaglandin dehydrogenase, 15 Hydroxyprostaglandin dehydrogenase [NAD (+) ], 15 hydroxyprostaglandin dehydrogenase [NAD+], 15 PGDH, 15-Hydroxyprostaglandin dehydrogenase, 15-Hydroxyprostaglandin dehydrogenase [NAD (+) ], 15-hydroxyprostaglandin dehydrogenase [NAD+], 15PGDH, 15-PGDH, AV026552, HPGD, PGDH, PGDH 1, PGDH1, PHOAR 1, PHOAR1, Prostaglandin dehydrogenase 1, Prostaglandin dehydrogenase1, SDR36C 1, SDR36C1, ATGD0137-10 µg, ATGD0137-20 µg, ATGD0137-50 µg, ATGD0137-100 µg, ATGD0137-250 µg, ATGD0137-500 µg, ATGD0137-1 mg, ATGD0137-010, ATGD0137-020, ATGD0137-050, ATGD0137-100, ATGD0137-250, ATGD0137-500, ATGD0137-01M

Store the plasmid at -20C.

Lyophilized

Immunology

601688

NP_000851.2

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_000860

801bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.