HQ-O RTD amyloid Plaque Stain Reagent

A novel zinc chelator, HQ-O, was developed for localizing zinc within amyloid plaques. The histology involves incubating tissue sections in a dilute aqueous solution of HQ-O.

HQ-O Ready-To-Dilute (RTD) Stain Reagent is designed to label amyloid plaques in paraffin-embedded or freshly cut frozen tissue sections.

HQ-O tracer

Human, Mouse, Other Mammals (Predicted), Rat

Amyloid plaque

To visualize the HQ-O tracer, it is recommended to use a filter cube designed for visualizing Fluorescein/FITC or a blue-light laser. Although it can be seen with both narrow and wide-band pass filters, there is no need to use a narrow band filter since the compound does not "bleed through" when excited with other filters. A recommended excitation range of a wide band filter is 447 - 503 nm, with a peak at 475 nm.

IHC-Frozen, IHC-Paraffin-embedded

10X

Purified

For research use only.

Shipped on ice packs

Store 10X stock solution at 2-8°C protected from light, for up to 6 months. The diluted dye (1X) should be used within 24 hours.

As a fluorescent zinc chelator, HQ-O is unique as it takes advantage of the known presence of concentrated zinc in amyloid plaques. Studies with HQ-O revealed that fluorescent plaque-like structures are only seen when synthetic Aβx-42 is aggregated in the presence of zinc. Under blue light excitation, plaque structures appear bright green fluorescent in the brain parenchyma, correlating closely with plaque structures observed following Aβ antibody staining.

Fluorescence

Liquid, ready to dilute

Species Independent