HSD3B7 Antilichaam / 3 bèta-hydroxysteroïde dehydrogenase type 7

This gene encodes an enzyme which is involved in the initial stages of the synthesis of bile acids from cholesterol and a member of the short-chain dehydrogenase/reductase superfamily. The encoded protein is a membrane-associated endoplasmic reticulum protein which is active against 7-alpha hydrosylated sterol substrates. Mutations in this gene are associated with a congenital bile acid synthesis defect which leads to neonatal cholestasis, a form of progressive liver disease. Multiple transcript variants encoding different isoforms have been found for this gene.

Western blot: 1-2 µg/mL, Immunofluorescence: 5 µg/mL, ELISA: 0.1-0.5 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

Q9H2F3

Human

An E.coli-derived human recombinant protein (amino acids E23-A363) was used as the immunogen for the HSD3B7 antibody.

WB, IF, ELISA, IHC-P

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This HSD3B7 antibody is available for research use only.

After reconstitution, the HSD3B7 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the HSD3B7 antibody should be determined by the researcher.

Cytoplasm (ER)

Immunofluorescent staining of FFPE human A549 cells with HSD3B7 antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.