Hsp70 Antilichaam / Warmteshock eiwit 70 / HSPA8

Western blot: 1:500-1:2000, Immunohistochemistry: 1:50-1:200, Immunocytochemistry/Immunofluorescence: 1:50-1:200, Immunoprecipitation: 1:50, Flow Cytometry: 1:50

P0DMV8, P0DMV9

Human

A synthesized peptide derived from human Hsp70 was used as the immunogen for the Hsp70 antibody.

31H87

WB, IHC, ICC, IF, IP, FACS

Affinity chromatography

Liquid

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

This Hsp70 antibody is available for research use only.

Store the Hsp70 antibody at -20°C.

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Optimal dilution of the Hsp70 antibody should be determined by the researcher.

Western blot analysis of Hsp70 using anti-Hsp70 antibody. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hsp70 antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. The predicted molecular weight of Hsp70 is ~70 kDa and ~64 kDa (two isoforms) .