HT1080 Lysaat

HT1080 is a fibrosarcoma cell line which has been used extensively in biomedical research. HT-1080 cell line are derived from tissue taken in a biopsy of a fibrosarcoma present in a 35 year-old male. The patient who supplied the sample had not undergone radio or chemotherapy and this lack of therapy is important as both radio and chemotherapy may introduce unwanted mutations into the sample.

Human

WB

2 mg/mL (determined by BCA assay)

HT1080, ATGL0032-10 µg, ATGL0032-20 µg, ATGL0032-50 µg, ATGL0032-100 µg, ATGL0032-250 µg, ATGL0032-500 µg, ATGL0032-1 mg, ATGL0032-10, ATGL0032-20, ATGL0032-50, ATGL0032-100, ATGL0032-250, ATGL0032-500, ATGL0032-1

Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.

Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)

Lysates

Human

All lanes : HT1080 Whole Cell Lysate (40ug) ; ; ; ; Lane 1. : Anti-GAPDH antibody (ATGA0181) ; ; Lane 2. : Anti-Beta tubulin antibody (ATGA0196) ;

Heat lysate to 95°C for 5 minutes and rapidly cooling before use.

Western Blot analyzed by both GAPDH and Beta tubulin antibody