HTT Antilichaam / Huntingtin

The huntingtin gene, also called HTT or HD (Huntington disease) gene, is the IT15 (interesting transcript 15) gene which codes for a protein called the huntingtin protein. It is mapped to 4p16.3. The protein has no sequence homology with other proteins and is highly expressed in neurons and tests in humans and rodents. HTT upregulates the expression of Brain Derived Neurotrophic Factor (BDNF) at the transcription level, and this gene is required for normal development. The HTT protein is involved in vesicle trafficking as it interacts with HIP1, a clathrin-binding protein, to mediate endocytosis, the absorption of materials into a cell. HTT was also visualized as punctate staining likely to represent nerve endings. What's more, wildtype HTT may function in the nucleus in the assembly of nuclear matrix-bound protein complexes involved with transcriptional repression and RNA processing.

Immunohistochemistry (FFPE) : 2-5 µg/mL, ELISA: 0.1-0.5 µg/mL

P42858

Human

An E.coli-derived human recombinant protein (amino acids R3079-C3142) was used as the immunogen for the HTT antibody.

IHC-P, ELISA

Antigen affinity chromatography

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This HTT antibody is available for research use only.

After reconstitution, the HTT antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the HTT antibody should be determined by the researcher.

Cytoplasm, Nucleus

IHC staining of FFPE human lung cancer tissue with HTT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.