Humaan ACAT1-antilichaam

ACAT1 (acetyl-Coenzyme A acetyltransferase 1) is a 417 amino acid protein. ACAT1 is a mitochondrial enzyme involved in the formation and degradation of ketone bodies and is necessary for the proper metabolic processing of isoleucine. ACAT1 and ACAT2 catalyze the formation of acetoacetyl-CoA from two acetyl-CoA molecules. These enzymes are also capable of the reverse reaction. Defects in ACAT1 are a cause of 3-ketothiolase deficiency. 3-ketothiolase deficiency is an inborn error of isoleucine catabolism characterized by intermittent ketoacidotic attacks associated with unconsciousness. Some patients die during an attack or are mentally retarded.

Acetyl-CoA acetyltransferase 1, ACAT, MAT, T2, THIL

Human

Human

Recombinant human ACAT1 (34-427aa) purified from E. coli

2C5

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

ACAT1, ATGA0240-10 µg, ATGA0240-20 µg, ATGA0240-50 µg, ATGA0240-100 µg, ATGA0240-250 µg, ATGA0240-500 µg, ATGA0240-1 mg, ATGA0240-10, ATGA0240-20, ATGA0240-50, ATGA0240-100, ATGA0240-250, ATGA0240-500, ATGA0240-1

Antonenkov, V.D., et al. (2000) Eur J Biochem 267: 2981-2990.; ; Korman, S.H. (2006) Mol Genet Metab 89: 289-299.; ; Yamaguchi, S., et al. (1988) J Clin Invest 81 (3) : 813-817.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Metabolism

NP_000010

P24752

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1. : Hep3B cell lysate; ; Lane 2. : 293T cell lysate; ; Lane 3. : A431 cell lysate {ATGA0240-WB2.jpg}

ICC/IF analysis of ACAT1 in Hep3B cells. The cell was stained with ATGA0240 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .