Humaan ACOT11 antilichaam

ACOT11 (Acyl-CoA thioesterase11), also known as BFIT, is a protein with acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates. Expression of this protein in mouse has been associated with obesity and alternative splicing results in two transcript variants encoding different isoforms.

Thioesterase, adipose associated isoform BFIT2, acyl-CoA thioesterase 11, thioesterase, adipose associated, STARD14, BFIT, KIAA0707, BFIT1, THEM1, StAR-related lipid transfer (START) domain containing 14

Human

Human

Recombinant human ACOT11 (19-250aa) purified from E. coli

J4B2

Unconjugated

ELISA, WB, IHC

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

ACOT11, AFA0903-10 µg, AFA0903-20 µg, AFA0903-50 µg, AFA0903-100 µg, AFA0903-250 µg, AFA0903-500 µg, AFA0903-1 mg, AFA0903-10, AFA0903-20, AFA0903-50, AFA0903-100, AFA0903-250, AFA0903-500, AFA0903-1

S. H. Adams, et al: (2001) Biochem. J. 360, 135-142.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Angiogenesis

NP_671517

Q8WXI4

The cell lysates of LNCap, Hep3B (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human ACOT11 (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Paraffin embedded sections of human colon cancer tissue were incubated with anti-human ACOT11 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)