Humaan ACOT8 antilichaam

Acyl-coenzyme A thioesterase 8 is an enzyme that in humans is encoded by the ACOT8 gene. The protein encoded by this gene is a peroxisomal thioesterase that appears to be involved more in the oxidation of fatty acids rather than in their formation. The encoded protein can bind to the human immunodeficiency virus-1 protein Nef, and mediate Nef-induced down-regulation of CD4 in T-cells. Multiple transcript variants encoding several different isoforms have been found for this gene.

Acyl-coenzyme A thioesterase 8, hACTE-III, HNAACTE, the, PTE-1, PTE-2, PTE1, PTE2

Human

Human

Recombinant human ACOT8 (1-319aa) purified from E. coli

AT4D10

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

ACOT8, ATGA0353-10 µg, ATGA0353-20 µg, ATGA0353-50 µg, ATGA0353-100 µg, ATGA0353-250 µg, ATGA0353-500 µg, ATGA0353-1 mg, ATGA0353-10, ATGA0353-20, ATGA0353-50, ATGA0353-100, ATGA0353-250, ATGA0353-500, ATGA0353-1

Jones JM., et al. (1999) J Biol Chem. 274 (14) : 9216-9223.; ; Liu LX., et al. (1997) J Biol Chem. 272 (21) : 13779-13785.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Cancer

NP_005460

O14734

The recombinant Human ACOT8 (10ng) and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACOT8 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant Human ACOT8; ; Lane 2.: Ramos cell lysate; ; Lane 3.: A431 cell lysate; ; Lane 4 : 293T cell lysate; ; Lane 5 : A549 cell lysate

Flow cytometry analysis of ACOT8 in CTLL-2 cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

ICC/IF analysis of ACOT8 in A549 cells. The cell was stained with ATGA0353 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .