Humaan Adenosine Kinase/ADK antilichaam

Adenosine kinase (ADK) is the key regulator of adenosine metabolism. Because of the manifold receptor-dependent actions of adenosine, tight regulation of adenosine levels is crucial. The intracellular and extracellular pools of adenosine are in dynamic exchange by equilibrative and concentrative nucleoside transporters, so extracellular concentrations of adenosine are regulated by interplay of theses transporters with intracellular and extracellular enzymes of adenosine metabolism. Thus, the extracellular concentration of adenosine is enhanced by inhibition of equilibrative nucleoside transporters such as S- (4-nitrobenzyl) -6-thioinosine and cannabidiol and, stimulation of extracellular ATP breakdown and inhibition of intracellular adenosine removal.

ADK, AK, adenosine kinase, adenosine 5'-phosphotransferase

Human

Human

Recombinant human ADK (22-362aa) purified from E. coli

AT4F8

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

Adenosine Kinase, ADK, ATGA0206-10 µg, ATGA0206-20 µg, ATGA0206-50 µg, ATGA0206-100 µg, ATGA0206-250 µg, ATGA0206-500 µg, ATGA0206-1 mg, ATGA0206-10, ATGA0206-20, ATGA0206-50, ATGA0206-100, ATGA0206-250, ATGA0206-500, ATGA0206-1

Mathews II , et al. (1998) . Biochemistry 1037 (45) :15607-20.; ; Lindberg B, Klenow H, Hansen K (1967) . J. Biol. Chem. 242 (3) : 350-6.; ; CAPUTTO R (1951) . J. Biol. Chem. 189 (2) : 801-14.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Signal Transduction

NP_006712

P55263

The cell lysates of HepG2 and 293T (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ADK (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Flow cytometry analysis of ADK in A549 cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

ICC/IF analysis of ADK in A549 cells. The cell was stained with ATGA0206 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .